Methodological bias associated with soluble protein recovery from soil
نویسندگان
چکیده
منابع مشابه
Recovery of soluble, active recombinant protein from inclusion bodies.
In summary, three conditions need to be fulfilled to successfully sequence directly on large DNA templates. First, the amount of DNA in the sequencing reaction must be in the range of 3–4 μg. In this way, more DNA molecules will be available for priming during the first cycles of the PCR. Second, the DNA must be highly pure because relatively large amounts of DNA must be added in the sequencing...
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DNA extraction bias is a frequently cited but poorly understood limitation of molecular characterizations of environmental microbial communities. To assess the bias of a commonly used soil DNA extraction kit, we varied the cell lysis protocol and conducted multiple extractions on subsamples of clay, sand, and organic soils. DNA, as well as bacterial and fungal ribosomal gene copies as measured ...
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Previous studies of prognostic factors of anorexia nervosa (AN) course and recovery have followed clinical populations after treatment discharge. This retrospective study examined the association between prognostic factors--eating disorder features, personality traits, and psychiatric comorbidity--and likelihood of recovery in a large sample of women with AN participating in a multi-site geneti...
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The problem of publication bias has long been discussed in research fields such as medicine. There is a consensus that publication bias is a reality and that solutions should be found to reduce it. In methodological computational research, including cancer informatics, publication bias may also be at work. The publication of negative research findings is certainly also a relevant issue, but has...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-29559-4